Skip to main content
Fig. 1 | Retrovirology

Fig. 1

From: Absolute quantification of HTLV-1 basic leucine zipper factor (HBZ) protein and its plasma antibody in HTLV-1 infected individuals with different clinical status

Fig. 1

Generation and characterization of anti-HTLV-1 bZIP factor (HBZ) monoclonal antibodies (mAbs). a HBZ protein levels in HBZ-transfected 293T cells were analyzed by indirect immunofluorescence using anti-HBZ mAbs P6-A7 (raised against peptide #1, mouse IgG2b), #20-H12 (raised against peptide #2, mouse IgG1), #91-1 (raised against peptide #3, rat IgG1), and #7-1 (raised against recombinant HBZ, mouse IgG2b). b HBZ expression vector-transfected 293T cells (HBZ) and mock-transfected 293T cells (Mock) were analyzed by western blotting using the novel anti-HBZ mAbs. c HBZ expression vector-transfected 293T cells (red line) and mock-transfected 293T cells (blue line) were analyzed by flow cytometry using the novel anti-HBZ mAbs. Three independent experiments were performed, and representative data are shown in each figure

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us