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Fig. 4 | Retrovirology

Fig. 4

From: HIV-1 capsid is involved in post-nuclear entry steps

Fig. 4

C-A1 reduces sensitivity to T5Cyp in CD4+ T cells. a CsA washout assays were performed on Jurkat cells stably expressing T5Cyp. Cells were infected with HIV-1GFP WT, N74D or A105S mutants in the presence of 1 μM CsA alone or in combination with 3 μM C-A1. The drugs were washed out at the indicated time points and cells analyzed by FACS 48 h post-infection to calculate the percentage of infected (GFP+) cells. Some background infection was detected after spinoculation hence values on the Y-axis do not start at 0. Data were plotted using XLfit to determine the half time of uncoating (ToU50). The representative ToU50 of six experiments is shown (red dashed line). b Plot showing average values of ToU50 ± SEM of four independent experiments. Each dot on the graph represents an independent experiment (note that five experiments were carried out with the A105S mutant virus). *p < 0.02, statistical significance was calculated using Student’s unpaired, two-tailed t test

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