Skip to main content
Fig. 1 | Retrovirology

Fig. 1

From: Combination of nanoparticle-based therapeutic vaccination and transient ablation of regulatory T cells enhances anti-viral immunity during chronic retroviral infection

Fig. 1

Foxp3+ CD4+ regulatory T cells down-regulate T cell responses after CaP nanoparticle vaccination. a Frequencies of Foxp3+ CD4+ regulatory T cells were determined by flow cytometry in naïve and chronically FV-infected mice 7 and 14 days post vaccination (d.p.v.) with functionalized CaP nanoparticles. Representative dot plots of the analysis are shown. b Ratios of fully activated CD4+ CD43+ or CD8+ CD43+ effector T cells (EFF) to Foxp3+ CD4+ T cells. c Frequencies of Ki67+ Foxp3+ CD4+ regulatory T cells. d Correlation of the percentage of GzmB expressing tetramer stained FV-specific CD8+ T cells to frequencies of Foxp3+ CD4+ T cells 14 d.p.v. with PBS (left) or functionalized CaP NPs (right). Combined results of three independent experiments are shown. Bars represent mean ± SEM. Statistical analysis was performed by student’s t test. *p < 0.05; **p < 0.01; ***p < 0.001

Back to article page