Fig. 1
PML-mediated restriction of HIV-1 and SIVmac infection in SupT1 cells. a WB analysis of human SupT1 cells stably transduced with shPMLs. Cells were stably transduced with either the control shRNA targeting luciferase or with shRNAs targeting all hPML isoforms. PML expression levels were analyzed by WB using a polyclonal antibody (upper panel). The same blot was reprobed with an anti-actin antibody as a loading control. The graph on the right shows the ratios of PML compared to actin following densitometry analysis. b Effects of shRNA-mediated depletion of hPML on HIV-1 and SIVmac infectivity. The cells stably expressing shPMLs or control shRNAs were infected with HIV-1NL-GFP (left) or SIVmac-GFP (right) (MOI of 0.1). Two days later, the percentages of infected cells were measured by FACS. The values represent the means of three independent experiments with standard deviations (**P < 0.01, two-tailed Student’s t test). c Effects of shRNA-mediated depletion of hPML on HIV-1 and SIVmac LTR-driven GFP expression. GFP MFI values are shown for the experiments in b (ns non-significant in the two-tailed Student’s t test)