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Fig. 8 | Retrovirology

Fig. 8

From: 2-thio-6-azauridine inhibits Vpu mediated BST-2 degradation

Fig. 8

Effect of 2-thio-6-azauridine on Vpu-induced ubiquitination of BST-2. 293T cells were co-transfected with plasmid pBST-2, pVphu and CW7, which express BST-2, Vpu and C-myc tagged ubiquitin respectively (1:2:4). 24 h post transfection, cells were treated with DMSO, 5 µM 2-thio-6-azauridine and/or 50 nM Concanamycin A for another 24 h. Cell lysates were then immunoprecipitated with rabbit BST-2 antibody and treated with PNGase F, which was used to remove N-glycans from BST-2, followed by immunoblotting with mouse C-myc and HA antibody for ubiquitin and BST-2 detection respectively. The normalized ubiquitination Level of BST-2 was shown as bar graph

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