Fig. 6

2-thio-6-azauridine dose not affect Vpu induced down-regulation of cell surface CD4 and K5 induced BST-2 degradation. a pMX hCD4 plasmid expressing human CD4 was transfected to HeLa or HeLa-Vpu cells, followed by treating the cells with 5 μM of 2-thio-6-azauridine at 24 h post transfection. The CD4-positive cells were scored by flow cytometry at 48 h post transfection. b KSHV K5 protein expressing plasmid pcDNA3.1-K5-HA was transfected to HeLa, followed by treating the cells with 5 μM of 2-thio-6-azauridine or 50 nM MG132 at 24 h post transfection. The cell surface BST-2 level was scored by flow cytometry at 48 h post transfection