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Fig. 3 | Retrovirology

Fig. 3

From: 2-thio-6-azauridine inhibits Vpu mediated BST-2 degradation

Fig. 3

2-thio-6-azauridine reduces HIV-1 particle release. a Plasmids pNL-Luc-E- and pHIT/G were co-transfected with either pBST-2 or the control vector into 293T cells. Cells were treated with DMSO or different concentration of 2-thio-6-azauridine (0.05, 0.5, 5 μM) at 24 h post transfection. The cells and viruses in the culture supernatant were harvested separately at 48 h post transfection and then the HIV-1 Gag p55 in cell lysate and HIV-1 Gag P24 in virions were detected by Western Blot. b By normalizing the Gag p55 level in virions, the P24 and VSV-G were detected by Western Blot

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