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Fig. 2 | Retrovirology

Fig. 2

From: 2-thio-6-azauridine inhibits Vpu mediated BST-2 degradation

Fig. 2

2-thio-6-azauridine inhibits HIV-1 in a BST-2-dependent manner. a Single-round pseudotyped HIV-1 expressing plasmids pNL-Luc-E- and pHIT/G were co-transfected with pBST-2 which expresses HA tagged BST-2 or the control vector to 293T cells. 24 h post transfection, the cells were treated with 5 μM of 2-thio-6-azauridine. Viruses in the culture supernatants were harvested at 48 h post transfection. Viral p24 and virus infectivity were measured as described in “Methods”. b Establishment of HeLa-shRNA-BST-2 cells, a BST-2 knockdown HeLa cell line. Western blot detection of BST-2 expression in HeLa and HeLa-shRNA-BST-2 cells is shown in the left panel. Results of relative infectivity assay using a single-round HIV-1 infection in HeLa and HeLa-shRNA-BST-2 cells are shown in the right panel. c HeLa and HeLa-shRNA-BST-2 cells were co-transfected with plasmids pNL-Luc-E- and pHIT/G to produce single-round HIV-1 particles. Cells were treated with 5 μM of 2-thio-6-azauridine 24 h post transfection. Viral p24 and virus infectivity measured as described in “Methods”. d The IC50 value of 2-thio-6-azauridine in HeLa and HeLa-shRNA-BST-2 cells. (*p < 0.05, t test)

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