Fig. 5

Increasing amounts of pseudotyped HIV-1 vector improve transduction efficiency but cannot completely overcome block. Primary human bone marrow derived and adult peripheral mobilized CD34+ cells were pre-incubated with Flt3 ligand, stem cell factor, and thrombopoietin for 24 h and then exposed to various amounts of virus. a Adult bone marrow derived CD34+ cells were exposed to virus at MOI through the range of 0–10 and evaluated by flow cytometry for expression of ZsGreen after 72 h with mean values and error bars indicating SEM (n = 3). b Adult bone marrow derived CD34+ cells were exposed to virus at MOI through the range of 0–100 and evaluated by flow cytometry for expression of ZsGreen after 72 h with mean values and error bars indicating SEM (n = 4). c Adult peripheral mobilized CD34+ cells were exposed to virus at MOI through the range of 0–10 and evaluated by flow cytometry for expression of ZsGreen after 72 h with mean values and error bars indicating SEM (n = 3). d Adult peripheral mobilized CD34+ cells were exposed to virus at MOI through the range of 0–100 and evaluated by flow cytometry for expression of ZsGreen after 72 h with mean values and error bars indicating SEM (n = 3)