Fig. 3

The kinetics of production of total viral DNA, 2-LTR circles, and integrated DNAs in adult human bone marrow derived CD34+ cells after exposure to pseudotyped HIV-1 vector at MOI of 1 and 10. Primary human CD34+ cells were incubated with either nevirapine (NVP) or with no cytokines (no cyt), Flt3 ligand (FLT3L), stem cell factor (SCF), Thrombopoietin (TPO), or all three cytokines (FST). 293T cells were similarly infected as a permissive cell control (293T). a Cells were lysed at 12, 24, or 48 h, after infection at MOI of 1, and DNA was extracted and analyzed by taqman PCR analysis for products relative to RNase-P (n = 3 per condition and per time point). DNA levels relative to RNase-P with bars representing mean and error bars indicating SEM. b Cells were analyzed at 12, 24, 48 and 72 h after infection at MOI of 10. Results are shown relative to RNase-P with bars representing mean and error bars indicating SEM. Asterisk indicates undetectable levels with NVP after amplification for 50 cycles