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Fig. 2 | Retrovirology

Fig. 2

From: Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integration

Fig. 2

Variable percentages of adult human marrow-derived CD34+ cells express ZsGreen after exposure to pseudotyped HIV-1 vector at relative MOI of 10 in the absence and with incubation with cytokines. Primary human CD34+ cells were incubated with no cytokines (no cyt), Flt3 ligand (FLT3L), stem cell factor (SCF), Thrombopoietin (TPO), all three cytokines (FST) or virus with nevirapine (NVP). 293T cells were similarly incubated with virus (293T + V) or virus plus nevirapine (293 + V + NVP). a Cells were evaluated by flow cytometry for expression of ZsGreen after 72 h and a representative example for each condition is shown for viral MOI of 1. b Percent CD34+ cells and 293T cells expressing ZsGreen with mean values and error bars indicating SEM (n = 3) for samples incubated with viral MOI of 1. c A representative example for CD34+ cells incubated in each condition is shown for viral MOI of 10. d Percent CD34+ cells and 293T cells expressing ZsGreen with mean values and error bars indicating SEM (n = 3) for samples incubated with virus at MOI of 10

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