Table 2 Annealing parameters of HIV-2 NC, MA, GagΔp6
From: The matrix domain contributes to the nucleic acid chaperone activity of HIV-2 Gag
HIV-2 protein (μM) | Annealing rate | NA annealed (%) |
|---|---|---|
TAR annealing (20Â mM NaCl) | ||
 NC (0.2) | 2.0 ± 0.40 | 95.3 ± 0.7 |
 MA (0.2) | 0.6 ± 0.05 | 91.1 ± 2.4 |
 GagΔp6 (0.2) | 0.9 ± 0.11 | 92.5 ± 1.3 |
tRNALys3 annealing (20Â mM NaCl) | ||
 NC (1.5) | 1.87 ± 0.44 | 95.1 ± 1.8 |
 MA (1.5) | 0.17 ± 0.08 | 37.8 ± 5.0 |
 GagΔp6 (1.5) | 0.18 ± 0.06 | 85.1 ± 5.2 |
tRNALys3 annealing (150Â mM NaCl) | ||
 NC (1.5) | 0.07 ± 0.03 | 35.8 ± 4.9 |
 MA (1.5) | 0.21 ± 0.06 | 48.1 ± 4.9 |
 GagΔp6 (1.5) | 0.26 ± 0.03 | 82.1 ± 3.4 |
 NC (3) | 0.11 ± 0.02 | 43 ± 5.2 |
 MA (3) | 0.22 ± 0.07 | 60 ± 4.9 |
 GagΔp6 (3) | 0.37 ± 0.07 | 90 ± 3.7 |
RNA dimerization (150Â mM NaCl) | ||
 NC (6) | 0.48 ± 0.10 | 49.2 ± 1.6 |
 GagΔp6 (6) | 0.15 ± 0.01 | 45.7 ± 2.2 |