Fig. 2

Robustness and specificity of combinatorial library approach in the selection of linear and conformational antigenic fragments by bnAbs with known epitope specificity. a, c Overlapping sequences of the positive yeast clones selected by bnAbs (PG09, 12A12, VRCPG04, 3BNC60, 3BNC117, 10E8, 4E10, and 2F5) and aligned to the original full-length P08-gp160 and P11-gp160 sequence used in the construction of combinatorial yeast library. Vertical dashed lines highlight the V1V2, V3V4V5, and MPER regions in which the epitopes of bnAbs are located. b, d The number of amino acid residues among the selected fragments along their corresponding positions in the P08 and P11 envelope glycoprotein. The dominant stretch of amino acid residues is numbered on the top of each graph according to HXB2 and the red vertical bars separate gp120 and gp41. The hypervarible regions V1–V5, interspersed conserved regions C1–C5 in gp120, immune dominant (ID), MPER and transmembrane domain (TM) in gp41 are also indicated