Fig. 5

Romidepsin and Prostratin do not increase HERV expression in U1s. The HERVs analysed were: HK2 env, HK2 pol, HERV-W env (syncytin-1) and HERV-FRD env (syncytin-2). The fold change in HERV expression following drug treatment was compared to the untreated control (lines show 95 % CI) and was calculated relative to GAPDH expression. Romidepsin and prostratin were used at final concentrations of 0.2 and 1 μM, respectively. The data points represent the relative fold change in expression normalised with GAPDH (lines show 95 % CI) in two independent experiments. A significant change of expression (i.e. higher than the untreated cells) would show the 95 % CI to be higher than and not overlap the dashed horizontal line which indicates 1× relative fold change (two-sided test)