Fig. 2

The HDAC inhibitors panobinostat and vorinostat do not increase HERV expression in J-LAT-8.4 cells. The HERVs analysed were: HK2 env, HK2 pol, HERV-W env (syncytin-1) and HERV-FRD env (syncytin-2). The fold change in HERV expression following drug treatment was compared to the untreated control (lines show 95 % CI) and was calculated relative to GAPDH expression. The doses of the drugs used were vorinostat (1 μM/well), panobinostat (0.1 μM/well), PMA (0.1 μg/μL). The data points (empty circles) represent the relative fold change in expression normalised with GAPDH (black squares show the median and orange lines show 95 % CI) for up to three replicates in four independent experiments. A significant change of expression (i.e. higher than the untreated cells) would show the 95 % CI to be higher than and not overlap the dashed horizontal line which indicates 1× relative fold change (two-sided test)