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Fig. 4 | Retrovirology

Fig. 4

From: A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis

Fig. 4

Maximum likelihood phylogenetic tree constructed using complete BLV genomic sequences. A maximum likelihood phylogenetic tree was constructed from complete genomic sequences of BLV strains from a total of 25 samples (submitted to GenBank nucleotide sequence database and assigned accession numbers LC080664, LC080670, LC080669, LC080671, LC080673, LC080665, LC080667, LC080674, LC080672, LC080675, LC080666, LC080668, LC080663, LC080659, LC080661, LC080662, LC080660, LC080654, LC080655, LC080651, LC080652, LC080653, LC080656, LC080657, LC080658) including one sample from Peru, seven from Paraguay and 17 from Bolivia, together with eight reference sequences obtained from the GenBank nucleotide sequence database (whole genomes of FLK-BLV pBLV913, LS1, LS2, LS3, Arg41, Arg38, Japan, and USA with accession numbers EF600696, HE967301, HE967302, HE967303, FJ914764, AF257515, K02120, and AF033818, respectively). Gaps/missing data were treated as partial deletions and, finally, 8374 of 8728 sites were used for the calculation. One thousand replications were performed for calculation of bootstrap values (indicated on the tree). Strains from this study are indicated in the tree by the sample ID together with the location of collection. Reference sequences in the tree are indicated by strain name. Genotypes are indicated by numbers to the right of the figure. South American BLV strains in this study are marked according to country of isolation as follows: Peru (filled triangle), Paraguay (filled circle), and Bolivia (filled diamond). Genotypes-1, -2, -4, -6, and -9 are shown in pastel blue, violet, blue, twilight violet and orange, respectively. The bar at the bottom of the figure denotes distance

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