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Fig. 3 | Retrovirology

Fig. 3

From: Enhancement of HIV-1 infection and intestinal CD4+ T cell depletion ex vivo by gut microbes altered during chronic HIV-1 infection

Fig. 3

HAMB induce low levels of LP CD4 T cell activation and proliferation. LPMC (n = 6) were pre-labeled with CFSE and exposed to High abundance or Low abundance HAMB species or to control bacteria (E. coli, B. infantis) (2.5 bacteria: 1 LPMC) for 5 days. LPMC were harvested and frequencies of activated (CD38+HLA-DR+, CD25+) and proliferating (CFSElo) LP CD4 T cells were evaluated using flow cytometry. a Representative gating strategy illustrating CD38+HLA-DR+, CD25+ and CFSE profiles of viable LP CD4 T cells in presence or absence of bacteria with gates established on media (CD25+, CFSE) or FMO controls (CD38/HLA-DR). b Percentages of LP CD4 T cells co-expressing CD38 and HLA-DR. FMO control values have been subtracted. c Percentages of CD4 LP T cells expressing CD25. d Percentages of CFSElo LP CD4 T cells. Values are shown as symbols representing each individual donor to highlight the 1 donor that exhibited unusually high responses to HAMB. Line indicates the median value. Statistical analysis was performed using the Wilcoxon matched–pairs signed rank test comparing percentages of activated or proliferating LP CD4 T cells induced in response to bacteria to no bacteria. *p < 0.05, #p = 0.06. Legend details the abbreviations used for each bacteria (x axis)

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