Skip to main content

Advertisement

Fig. 7 | Retrovirology

Fig. 7

From: Myristoylation drives dimerization of matrix protein from mouse mammary tumor virus

Fig. 7

Mutations L11N, F12N and V15N in MA domain (MA-3N) reduce extracellular MMTV particle production. a Pulse-chase analysis of immature MMTV particles produced in transfected 293T cells. Cells expressed wild-type MMTV (WT) and the virus with the mutation in MA domain of Gag (MA-3N); M mock-transfected cells. Cells were labeled for 1 h with [35S]methionine and [35S]cysteine (lanes 13) and then chased for 12 h (lanes 45). At both time points, cells were harvested, lysed, and subjected to immunoprecipitation. Released particles (lanes 67) were collected from the culture media by ultracentrifugation through a 20 % sucrose cushion. Viral proteins were immunoprecipitated with polyclonal rabbit anti-CA serum, separated on a 10 % SDS-PAGE gel, and subjected to phosphorimager analysis. b Comparative analysis of released particle production. The efficiency was expressed as a share of the signal from the released particulate Gag from the total amount of Gag synthesized. Mutant MA-3N is shown relative to the WT (set to 1). Each bar represents the average of results from three independent experiments, and standard error of the mean is shown

Back to article page