Table 4 Oligonucleotide primers for generation of pBLV-IF mutants
Mutant | Forward primer sequence | Reverse primer sequence |
|---|---|---|
R7A | gctCGCCGGTTTGGAGCCAG | agcCCCTTGTGGGGGGGGCCAGT |
R9A | CGCgctTTTGGAGCCAGGGCCA | AAAagcGCGCCGCCCTTGTGG |
F10A | CGCCGGgctGGAGCCAGGGCCA | agcCCGGCGCCGCCCTTGTGG |
V16A | GCCATGgctACATATGATTGCGAGCC | ATATGTagcCATGGCCCTGGCTCCAAACC |
Y18A | GTCACAgctGATTGCGAGCCCCGATGC | GCAATCagcTGTGACCATGGCCCTGGC |