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Table 4 Oligonucleotide primers for generation of pBLV-IF mutants

From: Identification and characterization of common B cell epitope in bovine leukemia virus via high-throughput peptide screening system in infected cattle

Mutant Forward primer sequence Reverse primer sequence
R7A gctCGCCGGTTTGGAGCCAG agcCCCTTGTGGGGGGGGCCAGT
R9A CGCgctTTTGGAGCCAGGGCCA AAAagcGCGCCGCCCTTGTGG
F10A CGCCGGgctGGAGCCAGGGCCA agcCCGGCGCCGCCCTTGTGG
V16A GCCATGgctACATATGATTGCGAGCC ATATGTagcCATGGCCCTGGCTCCAAACC
Y18A GTCACAgctGATTGCGAGCCCCGATGC GCAATCagcTGTGACCATGGCCCTGGC
  1. The sequences corresponding to alanine substitution are underlined