Skip to main content

Table 3 The comparison results of four cattle samples for a peptide microarray

From: Identification and characterization of common B cell epitope in bovine leukemia virus via high-throughput peptide screening system in infected cattle

No. Peptide sequence Cattleb no. No. Peptide sequence Cattleb no. No. Peptide sequence Cattleb no.
JB JB JB
S4 S6 N1 N2 S4 S6 N1 N2 S4 S6 N1 N2
p15a       p24a       gp51a      
1 MGNSPSYNPPAGISP + + 28 DLRSQYQNLWLQAWK + 12 RYTLDSVNGYPKIYW +
2 PSYNPPAGISPSDWL + 29 QYQNLWLQAWKNLPT 13 DSVNGYPKIYWPPPQ
3 PPAGISPSDWLNLVQ 30 LWLQAWKNLPTRPSV 14 GYPKIYWPPPQGRRR +
4 ISPSDWLNLVQSAQR + 31 AWKNLPTRPSVQPWS 15 IYWPPPQGRRRFGAR + + +
5 DWLNLVQSAQRLNPR + 32 LPTRPSVQPWSTIVQ + 16 PPQGRRRFGARAMVT + + + +
6 LVQSAQRLNPRPSPS 33 PSVQPWSTIVQGPAE + 17 RRRFGARAMVTYDCE + + +
7 AQRLNPRPSPSDFTD 34 PWSTIVQGPAESYVE 18 GARAMVTYDCEPRCP +
8 NPRPSPSDFTDLKNY 35 IVQGPAESYVEFVNR 19 MVTYDCEPRCPYVGA
9 SPSDFTDLKNYIHWF + 36 PAESYVEFVNRLQIS + 20 DCEPRCPYVGADRFD
10 FTDLKNYIHWFHKTQ + 37 YVEFVNRLQISLADN 21 RCPYVGADRFDCPHW
11 KNYIHWFHKTQKKPW 38 VNRLQISLADNLPDG + 22 VGADRFDCPHWDNAS
12 HWFHKTQKKPWTFTS 39 QISLADNLPDGVPKE + + + 23 RFDCPHWDNASQADQ
13 KTQKKPWTFTSGGPT 40 ADNLPDGVPKEPIID + 24 PHWDNASQADQGSFY
14 KPWTFTSGGPTSCPP 41 PDGVPKEPIIDSLSY 25 NASQADQGSFYVNHQ
15 FTSGGPTSCPPGRFG 42 PKEPIIDSLSYANAN + 26 ADQGSFYVNHQILFL
16 GPTSCPPGRFGRVPL 43 IIDSLSYANANKECQ + 27 SFYVNHQILFLHLKQ
17 CPPGRFGRVPLVLAT 44 LSYANANKECQQILQ + 28 NHQILFLHLKQCHGI
18 RFGRVPLVLATLNEV + 45 NANKECQQILQGRGL + 29 LFLHLKQCHGIFTLT
19 VPLVLATLNEVLSNE 46 ECQQILQGRGLVAAP 30 LKQCHGIFTLTWEIW +
20 LATLNEVLSNEGGAP + + 47 ILQGRGLVAAPVGQK + 31 HGIFTLTWEIWGYDP + +
21 NEVLSNEGGAPGASA 48 RGLVAAPVGQKLQAC 32 TLTWEIWGYDPLITF + +
22 SNEGGAPGASAPEEQ 49 AAPVGQKLQACAHWA 33 EIWGYDPLITFSLHK
23 GAPGASAPEEQPPPY + 50 GQKLQACAHWAPKMK 34 YDPLITFSLHKIPDP +
24 ASAPEEQPPPYDPPA 51 QACAHWAPKMKQPAI 35 ITFSLHKIPDPPQPD +
25 EEQPPPYDPPAI + 52 HWAPKMKQPAIL 36 LHKIPDPPQPDFPQL
p24a       p12a       37 PDPPQPDFPQLNSDW +
           38 QPDFPQLNSDWVPSV
1 LPIISEGNRNRHRAW + + 1 VHTPGPKMPGPRQPA + + + 39 PQLNSDWVPSVRSWA
2 SEGNRNRHRAWALRE + + 2 GPKMPGPRQPAPKRP 40 SDWVPSVRSWALLLN + +
3 RNRHRAWALRELQDI + + + 3 PGPRQPAPKRPPPGP 41 PSVRSWALLLNQTAR
4 RAWALRELQDIKKEI + + + 4 QPAPKRPPPGPCYRC 42 SWALLLNQTARAFPD +
5 LRELQDIKKEIENKA + + + 5 KRPPPGPCYRCLKEG 43 LLNQTARAFPDCAIC
6 QDIKKEIENKAPGSQ + + 6 PGPCYRCLKEGHWAR 44 TARAFPDCAICWEPS
7 KEIENKAPGSQVWIQ + + 7 YRCLKEGHWARDCPT 45 FPDCAICWEPSPPWA
8 NKAPGSQVWIQTLRL + 8 KEGHWARDCPTKATG + + + 46 AICWEPSPPWAPEIL
9 GSQVWIQTLRLAILQ 9 WARDCPTKATGPPPG + + + 47 EPSPPWAPEILVYNK
10 WIQTLRLAILQADPT 10 CPTKATGPPPGPCPI + + 48 PWAPEILVYNKTISS
11 LRLAILQADPTPADL + 11 ATGPPPGPCPICKDP 49 EILVYNKTISSSGPG
12 ILQADPTPADLEQLC + 12 PPGPCPICKDPSHWK + + + 50 YNKTISSSGPGLALP
13 DPTPADLEQLCQYIA + 13 CPICKDPSHWKRDCP 51 ISSSGPGLALPDAQI
14 ADLEQLCQYIASPVD + 14 KDPSHWKRDCPTLKS + + 52 GPGLALPDAQIFWVN + +
15 QLCQYIASPVDQTAH + + gp51a       53 ALPDAQIFWVNTSSF
16 YIASPVDQTAHMTSL +       54 AQIFWVNTSSFNTTQ +
17 PVDQTAHMTSLTAAI 1 TWRCSLSLGNQQWMT 55 WVNTSSFNTTQGWHH
18 TAHMTSLTAAIAAAE 2 SLSLGNQQWMTAYNQ 56 SSFNTTQGWHHPSQR +
19 TSLTAAIAAAEAANT + + 3 GNQQWMTAYNQEAKF 57 TTQGWHHPSQRLLFN +
20 AAIAAAEAANTLQGF 4 WMTAYNQEAKFSISI + 58 WHHPSQRLLFNVSQG
21 AAEAANTLQGFNPQN + 5 YNQEAKFSISIDQIL 59 SQRLLFNVSQGNALL
22 ANTLQGFNPQNGTLT + 6 AKFSISIDQILEAHN + 60 LFNVSQGNALLLPPI +
23 QGFNPQNGTLTQQSA + 7 ISIDQILEAHNQSPF + 61 SQGNALLLPPISLVN +
24 PQNGTLTQQSAQPNA + 8 QILEAHNQSPFCAKS + 62 ALLLPPISLVNLSTA +
25 TLTQQSAQPNAGDLR + 9 AHNQSPFCAKSPRYT 63 PPISLVNLSTASSAP
26 QSAQPNAGDLRSQYQ + 10 SPFCAKSPRYTLDSV 64 LVNLSTASSAPPTRV +
27 PNAGDLRSQYQNLWL + 11 AKSPRYTLDSVNGYP 65 STASSAPPTRVRR
  1. Italic indicates a common B cell epitope which was identified in four BLV experimentally infected Japanese Black cattle by peptide microarray
  2. + only positive response with BLV positive serum; − none response with serums
  3. aAntigens were derived from BLV Gag (p15, p24, and p12) and Env gp51 proteins
  4. bThese BLV positive serums were obtained from BLV-experimentally infected cattle as shown in Table 1