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Table 2 The serums used in a new peptide ELISA high-throughput system

From: Identification and characterization of common B cell epitope in bovine leukemia virus via high-throughput peptide screening system in infected cattle

Local Farm no. BLV positive samplesa BLV negative samplesb
Cattle no. BoLA-DRB3 Cattle no. BoLA-DRB3
No. Alleles No. Alleles
Aichi 2 32 12 0101, 0201, 0502, 0503, 0701, 0902, 1001, 1101, 14011, 1501, 1601, 1801 5 6 0201, 0503, 1101, 1302, 1501, 1601
Ishikawa 1 12 7 0101, 0201, 0902, 1001, 1101, 1201, 1501 3 5 0601, 1101, 1201, 1501, 2703
Iwate 2 47 14 0101, 0201, 0502, 0503, 0701, 0902, 1001, 1101, 1201, 14,011, 1501, 1601, 2703, 4401 14 7 0503, 1001, 1101, 1201, 14011, 1501, 1601
Oita 2 30 13 0101, 0201, 0301, 0503, 0701, 0801, 0902, 1001, 1101, 1302, 1501, 1601, 2703 8 7 0101, 0201, 0301, 1001, 1101, 1201, 2703
Osaka 2 52 12 0101, 0201, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801, 2703 2 4 0101, 1101, 14011, 1501
Okinawa 2 43 12 0101, 0201, 0502, 0503, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1601 19 10 0101, 0201, 0503. 0701, 1001, 1101, 1201, 14011, 1501, 1601
Kagoshima 2 9 7 0502, 0503, 0701, 1001, 1302, 1501, 1601 28 10 0101, 0502, 0503, 0801, 1001, 1101, 1201, 1302, 14011, 1501, 1601, 2703
Kanagawa 2 55 11 0101, 0201, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1801, 2703 10 8 0101, 0902, 1001, 1101, 1201, 14011, 1501, 2703
Gifu 3 67 17 0101, 0201, 0501, 0502, 0503, 0701, 0801, 0902, 1001, 1101, 1201, 1302, 14011, 1501, 1601, 1801, 2703 1 2 0101, 1001
Nagano 2 14 12 0101, 0501, 0502, 0901, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801 7 2 0101, 0902, 1001, 1101, 14011, 1601, 2703
Shiga 2 24 10 0101, 0601, 0902, 1001, 1101, 1201, 14011, 1501, 1801, 2703 5 6 0101, 1001, 1101, 1201, 14011, 2703
Shizuoka 3 47 12 0101, 0201, 0503, 0901, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 2703 13 10 0101, 0502, 0503, 0701, 0902, 1001, 1101, 1201, 1501, 1601
Hyogo 2 37 11 0101, 0201, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801, 2703 30 10 0101, 0201, 0901, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801, 2703
Chiba 2 45 13 0101, 0201, 0601, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1701, 1801, 2703 16 12 0101, 0601, 0701, 0902, 1001, 1101, 1103, 14011, 1501, 1701, 2703, 3201
Tokyo 1 16 6 0101, 1001, 1101, 14011, 1501, 1801 15 10 0101, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801, 2703
Nagasaki 2 22 11 0101, 0503, 0601, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1601 43 16 0101, 0201, 0502, 0503, 0601, 0701, 0801, 0902, 1001, 1101. 1201, 14011, 1501, 1601, 2703, 3401
Niigata 2 42 15 0101, 0502, 0503, 0504, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 2002, 2703, 3401 15 14 0101, 0502, 0503, 0701, 0902, 1001, 1101, 1103, 1201, 1302, 14011, 1501 1601, 2802
Hokkaido 1 9 9 0101, 0201, 0502, 0503, 1001, 1201, 14011, 1601, 3401 5 6 0502, 0503, 1001, 1201, 1501, 1601
Mie 1 3 4 0101, 1001, 1101, 1501 20 11 0101, 0201, 0503, 1001, 1101, 1201, 1302, 14011, 1501, 1601, 1701
Yamagata 2 41 10 0101, 0502, 0503, 0701, 1001, 1201, 14011, 1501, 1601, 2703 11 9 0201, 0502, 0701, 0801, 1001, 1101, 1201, 1501, 1601
Total 38 647    270   
  1. Serum samples were obtained from 647 BLV naturally infected cattle which were positive for anti-BLV antibodies and BLV provirus as determined by an anti-BLV ELISA kit and a BLV-CoCoMo-qPCR-2 assay, respectively, and 270 BLV non-infected cattle
  2. aThese BLV positive samples had 25 different BoLA-DRB3 alleles that were typed by PCR-SBT method
  3. bThese BLV negative samples had 23 different BoLA-DRB3 alleles, which 20 alleles of them were same with identification alleles on BLV positive samples as described above (b)