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Table 2 The serums used in a new peptide ELISA high-throughput system

From: Identification and characterization of common B cell epitope in bovine leukemia virus via high-throughput peptide screening system in infected cattle

Local

Farm no.

BLV positive samplesa

BLV negative samplesb

Cattle no.

BoLA-DRB3

Cattle no.

BoLA-DRB3

No.

Alleles

No.

Alleles

Aichi

2

32

12

0101, 0201, 0502, 0503, 0701, 0902, 1001, 1101, 14011, 1501, 1601, 1801

5

6

0201, 0503, 1101, 1302, 1501, 1601

Ishikawa

1

12

7

0101, 0201, 0902, 1001, 1101, 1201, 1501

3

5

0601, 1101, 1201, 1501, 2703

Iwate

2

47

14

0101, 0201, 0502, 0503, 0701, 0902, 1001, 1101, 1201, 14,011, 1501, 1601, 2703, 4401

14

7

0503, 1001, 1101, 1201, 14011, 1501, 1601

Oita

2

30

13

0101, 0201, 0301, 0503, 0701, 0801, 0902, 1001, 1101, 1302, 1501, 1601, 2703

8

7

0101, 0201, 0301, 1001, 1101, 1201, 2703

Osaka

2

52

12

0101, 0201, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801, 2703

2

4

0101, 1101, 14011, 1501

Okinawa

2

43

12

0101, 0201, 0502, 0503, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1601

19

10

0101, 0201, 0503. 0701, 1001, 1101, 1201, 14011, 1501, 1601

Kagoshima

2

9

7

0502, 0503, 0701, 1001, 1302, 1501, 1601

28

10

0101, 0502, 0503, 0801, 1001, 1101, 1201, 1302, 14011, 1501, 1601, 2703

Kanagawa

2

55

11

0101, 0201, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1801, 2703

10

8

0101, 0902, 1001, 1101, 1201, 14011, 1501, 2703

Gifu

3

67

17

0101, 0201, 0501, 0502, 0503, 0701, 0801, 0902, 1001, 1101, 1201, 1302, 14011, 1501, 1601, 1801, 2703

1

2

0101, 1001

Nagano

2

14

12

0101, 0501, 0502, 0901, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801

7

2

0101, 0902, 1001, 1101, 14011, 1601, 2703

Shiga

2

24

10

0101, 0601, 0902, 1001, 1101, 1201, 14011, 1501, 1801, 2703

5

6

0101, 1001, 1101, 1201, 14011, 2703

Shizuoka

3

47

12

0101, 0201, 0503, 0901, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 2703

13

10

0101, 0502, 0503, 0701, 0902, 1001, 1101, 1201, 1501, 1601

Hyogo

2

37

11

0101, 0201, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801, 2703

30

10

0101, 0201, 0901, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801, 2703

Chiba

2

45

13

0101, 0201, 0601, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1701, 1801, 2703

16

12

0101, 0601, 0701, 0902, 1001, 1101, 1103, 14011, 1501, 1701, 2703, 3201

Tokyo

1

16

6

0101, 1001, 1101, 14011, 1501, 1801

15

10

0101, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 1801, 2703

Nagasaki

2

22

11

0101, 0503, 0601, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1601

43

16

0101, 0201, 0502, 0503, 0601, 0701, 0801, 0902, 1001, 1101. 1201, 14011, 1501, 1601, 2703, 3401

Niigata

2

42

15

0101, 0502, 0503, 0504, 0701, 0902, 1001, 1101, 1201, 14011, 1501, 1601, 2002, 2703, 3401

15

14

0101, 0502, 0503, 0701, 0902, 1001, 1101, 1103, 1201, 1302, 14011, 1501 1601, 2802

Hokkaido

1

9

9

0101, 0201, 0502, 0503, 1001, 1201, 14011, 1601, 3401

5

6

0502, 0503, 1001, 1201, 1501, 1601

Mie

1

3

4

0101, 1001, 1101, 1501

20

11

0101, 0201, 0503, 1001, 1101, 1201, 1302, 14011, 1501, 1601, 1701

Yamagata

2

41

10

0101, 0502, 0503, 0701, 1001, 1201, 14011, 1501, 1601, 2703

11

9

0201, 0502, 0701, 0801, 1001, 1101, 1201, 1501, 1601

Total

38

647

  

270

  
  1. Serum samples were obtained from 647 BLV naturally infected cattle which were positive for anti-BLV antibodies and BLV provirus as determined by an anti-BLV ELISA kit and a BLV-CoCoMo-qPCR-2 assay, respectively, and 270 BLV non-infected cattle
  2. aThese BLV positive samples had 25 different BoLA-DRB3 alleles that were typed by PCR-SBT method
  3. bThese BLV negative samples had 23 different BoLA-DRB3 alleles, which 20 alleles of them were same with identification alleles on BLV positive samples as described above (b)