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Table 1 Japanese black cattle examined in peptide microarray

From: Identification and characterization of common B cell epitope in bovine leukemia virus via high-throughput peptide screening system in infected cattle

Animal no.a BoLA-DRB3b alleles A; B Age (month) Sex WBCsc (cells/μl) PBLsd (cells/μl) Proviral loade (copies per 105 cells) Antibodies to BLVf
BLV-challenged BLV-challenged BLV-challenged BLV-challenged
Before After Before After Before After Before After
JBS4 1601; 1601 8 10,200 15,500 6560 11,100 0 67,284 +
JBS6 1601; 1601 6 9170 13,900 4690 9570 0 93,456 +
JBN1 1501; 2703 6 9060 8250 3084 4900 0 149 +
JBN2 1501; 0503 6 11,800 12,100 8142 9140 0 58,176 +
  1. BLV negative serum; + BLV positive serum
  2. aFour BLV-negative Japanese Black calves (6–8-months-old) were experimentally challenged intravenously with white blood cells containing a proviral load of 4 × 107 proviral copies, as determined by CoCoMo-qPCR-2 assay
  3. b BoLA-DRB3 alleles were genotyped by PCR-SBT method and are according to the BoLA nomenclature committee of the Immune Polymorphism Database (IPD)-MHC database. Both alleles, A and B, for each experimental animal was shown
  4. cWhite blood cells (WBCs) were measured at a single time point in all calves during the experiment
  5. dPeripheral blood lymphocytes (PBLs) were measured at a single time point in all calves during the experiment
  6. eProviral load (expressed as the number of copies of provirus per 105 peripheral blood mononuclear cells) was evaluated using CoCoMo-qPCR-2 assay
  7. fELISA (enzyme-linked immunosorbent assay) to detect anti-BLV antibodies was performed using the BLV ELISA kit