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Table 1 Japanese black cattle examined in peptide microarray

From: Identification and characterization of common B cell epitope in bovine leukemia virus via high-throughput peptide screening system in infected cattle

Animal no.a

BoLA-DRB3b alleles A; B

Age (month)

Sex

WBCsc (cells/μl)

PBLsd (cells/μl)

Proviral loade (copies per 105 cells)

Antibodies to BLVf

BLV-challenged

BLV-challenged

BLV-challenged

BLV-challenged

Before

After

Before

After

Before

After

Before

After

JBS4

1601; 1601

8

10,200

15,500

6560

11,100

0

67,284

+

JBS6

1601; 1601

6

9170

13,900

4690

9570

0

93,456

+

JBN1

1501; 2703

6

9060

8250

3084

4900

0

149

+

JBN2

1501; 0503

6

11,800

12,100

8142

9140

0

58,176

+

  1. BLV negative serum; + BLV positive serum
  2. aFour BLV-negative Japanese Black calves (6–8-months-old) were experimentally challenged intravenously with white blood cells containing a proviral load of 4 × 107 proviral copies, as determined by CoCoMo-qPCR-2 assay
  3. b BoLA-DRB3 alleles were genotyped by PCR-SBT method and are according to the BoLA nomenclature committee of the Immune Polymorphism Database (IPD)-MHC database. Both alleles, A and B, for each experimental animal was shown
  4. cWhite blood cells (WBCs) were measured at a single time point in all calves during the experiment
  5. dPeripheral blood lymphocytes (PBLs) were measured at a single time point in all calves during the experiment
  6. eProviral load (expressed as the number of copies of provirus per 105 peripheral blood mononuclear cells) was evaluated using CoCoMo-qPCR-2 assay
  7. fELISA (enzyme-linked immunosorbent assay) to detect anti-BLV antibodies was performed using the BLV ELISA kit