Fig. 2

Gene Ontology classification of differentially expressed genes in CCR4⁺CCR6⁺ Th17 versus CXCR3⁺CCR6⁻ Th1 cells. Shown are heatmaps of differentially expressed genes (p < 0.05, FC cut-off 1.3) selected for their biological functions as follows: a Cytokines (KEGG), b TCR signalling (reactome), and c transcription regulators (ingenuity). Coloring of the cells is scaled by the z score of each microarray probe individually. Results correspond to matched Th17 and Th1 subsets from n = 5 different HIV-uninfected donors isolated and stimulated by CD3/CD28 Abs for 3 days as described in Fig. 1