Fig. 3
Anti-OPN mAbs suppressed the tumor growth and metastasis of ATL cells inoculated into NOG mice. Six-week-old female NOG mice were subcutaneously inoculated with 2 × 107 TL-OmI cells. At 24 days after cell inoculation, the indicated anti-OPN mAbs (n = 5) or control IgG (n = 5) were intraperitoneally administered into the mice every 3–4 days. a Blood from the tail vein was collected for measurement of the mouse OPN level in the plasma. b The tumor size was measured every 3–4 days. c Immunohistochemical detection of tumor proliferation assessed by Ki-67 staining on day 40 after cell inoculation. d Blood from the tail vein was collected and metastatic cells were quantified by the number of tax genes assessed using qPCR. e Immunohistochemical detection of tumor metastasis by CD25 staining of liver tissue on day 40. f Immunohistochemical detection of CAFs by FAP staining of tumor tissues on day 40. For all of a–f, bars indicate mean values ± SEM. Statistically significant differences are shown as P values (*P < 0.05, **P < 0.01). NS no significant difference. For a, b and d, open squares, control IgG; filled squares, OPN RGD motif-recognizing mAb; open circles, OPN SVVYGLR motif-recognizing mAb; filled circles, OPN SVVYGLR motif-recognizing + RGD motif-recognizing mAbs