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Fig. 2 | Retrovirology

Fig. 2

From: Osteopontin-integrin interaction as a novel molecular target for antibody-mediated immunotherapy in adult T-cell leukemia

Fig. 2

Host-derived OPN production correlates with pathogenicity in tumor cell-inoculated NOG mice. Six-week-old female NOG mice were subcutaneously inoculated with 5 × 107 SLB-1 (n = 5), TL-OmI (n = 5), or 43Tb(−) (n = 5) cells. a The tumor size was measured every 3–4 days and is expressed in cubic millimeters. Bars indicate mean values ± SEM. b Survival rate was monitored based on death of the mice. c Blood was collected from the tail vein for measurement of the mouse OPN level in the plasma, and d for detection of metastatic cells as quantified by the number of tax genes assessed using qPCR. Bars indicate mean values ± SEM. For a–d, filled squares, SLB-1; filled circles, TL-OmI; filled triangles, 43Tb(−). e PBMCs were isolated on day 20 after the indicated cell inoculation and were stained with Giemsa. Scale bars, 50 μm. f Immunohistochemical staining of a subcutaneous tumor from an NOG mouse 20–50 days after the indicated cell inoculation. The sections were stained with Abs to the indicated proteins or with H&E. Arrows indicate the positive staining of mouse OPN. Scale bars, 100 μm. g Immunohistochemical staining of the lung from an NOG mouse 20–50 days after the indicated cell inoculation. The sections were stained with Abs to the indicated proteins or with H&E. Scale bars, 100 μm

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