Fig. 5

p2-peptide increased intracellular ATP content in MDMs. a Cell morphology and expression of surface receptors on MDMs. Freshly prepared MDMs were stained with monoclonal antibodies specific for CD14 (MAb M5E2), CD4 (MAb OKT4), or CCR5 (MAb 45531.111) and analyzed by flow cytometry. b Comparison of intracellular ATP level between MT-4 and MDMs. c The infection with the CCR5-tropic HIV_{AD8 WT} virus increased the intracellular ATP content in MDMs. In contrast, the intracellular ATP content after HIV_{NL-CH(AD8) p2(Q6A)} infection was not significantly increased compared with mock treatment. **p < 0.01, Nonrepeated measures ANOVA and Dunnett’s test. The mean values of at least three independent experiments are shown. d p2(Q6A) mutant virus showed a significant decrease in the level of the late viral cDNA product in MDMs as compared with that in the WT virus. MDMs were infected with VSV-G-pseudotyped HIV_{NL-CHΔenv p2(Q6A)}. Reverse transcription products were determined by quantitative PCR analysis using late-stage primers (R/gag), as described in “Methods”. To normalize the amount of cellular DNA (100 ng) in the samples, a primer pair complementary to the first exon of the human β-actin gene was used