Fig. 4

p2-peptide increased intracellular ATP content in MT-4 cells. (a, upper figure) The p2(Q6A) mutation did not affect normal CA processing. (a, lower figure) The entry efficiency of the p2(Q6A) mutant virus was determined in terms of the amount of CA protein released into the cytosolic fraction of MT-4 cells (see “Methods”). b The infection with the HIV_{NL-CH WT} virus increased the intracellular ATP content in MT-4 cells. In contrast, the intracellular ATP content after HIV_{NL-CH p2(Q6A)} infection was not efficiently increased. **p < 0.01, Nonrepeated measures ANOVA and Dunnett’s test. The mean values of at least three independent experiments are shown. c p2(Q6A) mutant virus showed a significant decrease in the level of the late viral cDNA product in MT-4 cells as compared with that in the WT virus. Reverse transcription products were determined by quantitative PCR analysis using late-stage primers (R/gag), as described in “Methods”. To normalize the amount of cellular DNA (100 ng) in the samples, a primer pair complementary to the first exon of the human β-actin gene was used