Fig. 4

Reverse transcription and expression of MLV genome in cells infected in absence of microtubules. a Detection of reverse transcription products by PCR in interphase and mitotic cells. Low molecular DNA was extracted from interphase and mitotic cells at the indicated hours postinfection. Primers derived from matrix-capsid region or from cytochrome B gene were used to amplify gDNA or mtDNA, respectively. Shown are the PCR products, separated by gel electrophoresis. b Unsynchronized (Interphase) or nocodazole-arrested (Mitotic) cells were infected with MLV virions that co-packaged the pQCXIP-GFP-C1 vector. The cells were allowed to cycle, beginning at 6 h post infection, and 2 days later, the percentage of GFP-positive cells was determined by FACS. Bars represent the mean ± standard error of the means (n = 3)