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Fig. 3 | Retrovirology

Fig. 3

From: Dynamics and restriction of murine leukemia virus cores in mitotic and interphase cells

Fig. 3

Viral cores reach and dock to mitotic chromosomes in absence of polymerized microtubules. a Timeline for infection during mitotic arrest. U/R (b) or NIH3T3 (c) cells expressing EMTB-mCherry (red) were treated with 2ME2 (b) or nocodazole (c), infected with wt GFP virions. The left images in (b) and (c) show drug-treated cells, not arrested at mitosis; while the other images in each panel show a representative arrested cell, imaged at the indicated times postinfection. GFP-labeled MLV cores are in green and condensed chromosomes appear as intracellular dark regions. Kymographs of selected time points [45 min (b; Additional file 12: Mov. S8) or 1 h (c)] show immobile MLV cores (appear as continuous green lines [15]), docked to mitotic chromosomes (examples are indicated by empty arrowheads). Scale bars 10 µm

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