Fig. 1

Entry and intracellular trafficking of MLV cores in interphase cells. Unsynchronized U/R/EMTB-mCherry cells were infected with DiD-labeled wt GFP virions and interphase cells were imaged at 5 min (a), 75 min (b, c) and 24 h (d) postinfection. All panels are single optical slices obtained by spinning-disc confocal microscopy. Panel c shows magnified time composites of insets in (b), prepared from Additional file 1: Mov. S1. Viral cores are in green; DiD signal and EMTB-marked microtubules are pseudocolored in red and blue, respectively. Full arrowheads mark GFP⁺DiD⁺ particles in a single confocal plane. In time composite images, empty arrowheads mark paths of GFP⁺DiD⁺ particles overlapping microtubules; ellipses enclose paths of GFP⁺DiD⁻ cores presenting minimal displacement and not localized to microtubules. Scale bars 10 µm