Fig. 7From: Identification and characterization of a new type of inhibitor against the human immunodeficiency virus type-1 nucleocapsid proteinA1752 induces abnormal HIV-1 core stability. a, b The virus particles produced from HIV-1 proviral plasmid-transfected 293FT cells were treated with A1752 and permeabilized either by Melittin (a) or Triton X-100 (b) at room temperature for 10 min and then exposed to a 37 °C for 30 min to disassemble the HIV-1 core structure. The resulting viruses were fractionated to a pellet and supernatant by centrifugation as described in “Methods”, and subjected to western blot analysis with anti-CA (a) or anti-RT (b) antibodiesBack to article page