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Fig. 4 | Retrovirology

Fig. 4

From: Identification and characterization of a new type of inhibitor against the human immunodeficiency virus type-1 nucleocapsid protein

Fig. 4

Effect of A1752 on infectivity of viruses generated by proviral DNA transfection and lentiviral system. The 293FT cells were transfected with a pNL4-3EGFP HIV-1-proviral DNA in the presence of the indicated inhibitors. a Post-transfection 48 h, EGFP expression within cells and viral production were analyzed, respectively. b An equal amount (2.5 ng in p24) of the virions produced in each case in (a) was taken to infect to MT-4 cells as described in Fig. 3, and the infection was analyzed using both a fluorescence microscopic observation of EGFP expression and quantitative FACS analysis (% of GFP) 48 h after infection. c 293FT cells were transfected with a pLenti/EGFP transgene plasmid and packaging plasmids (pLP1, pLP2, and pLP/VSVG) in the presence of the inhibitors indicated (middle column) The same amounts (10 ng in p24) of lentiviral vectors produced under the conditions were subjected to infection to fresh MT-4 cells and followed by determination of level of EGFP expression (the last column), and d HT1080 colony assay for the determination of the lentiviral vector infectivity. The colony forming units (CFU) were compensated with p24 ELISA values for the virus particles used in each case. Data are the mean ± SEM of three separate experiments

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