Skip to main content

Table 2 Lack of HIV-1 replication in PHA-activated CD4+T lymphocytes challenged with different exosome preparations

From: Latent HIV-1 is activated by exosomes from cells infected with either replication-competent or defective HIV-1

Days post-challenge input

CAp24 pg/ml

% of HIV-1+ cells

2

5

7

2

5

7

Exo U937

<15

<15

<15

<1

<1

<1

Exo U937 Δnef HIV-1

31

<15

<15

<1

<1

<1

Exo U937 Δnef

HIV-1 + wtNef

26

<15

<15

<1

<1

<1

Exo U937 Δnef

HIV-1 + NefG2A

44

<15

<15

<1

<1

<1

Exo CD4+ T lymphocytes

<15

<15

<15

<1

<1

<1

Exo CD4+ T lymphocytes + HIV-1

<15

<15

<15

<1

<1

<1

Exo MDMs

<15

<15

<15

<1

<1

<1

Exo MDMs + HIV-1

<15

<15

<15

<1

<1

<1

Exo Hut-78

<15

<15

<15

<1

<1

<1

Exo Hut-78/F12

89

<15

<15

<1

<1

<1

HIV-1, 50 pg

<15

9980

2400

<1

32.2

7.9

  1. A total of 5 × 104 CD4+ T lymphocytes were spinoculated with 120 μU of the exosome preparations indicated in the left column. As a control, the same number of cells was challenged with 50 pg CAp24 equivalents of the T-tropic NL4-3 HIV-1 strain. Cell cultures were washed 24 h later, and viral replication was monitored at the indicated days post-infection by measuring CAp24 levels in supernatants as well as scoring HIV-1 expressing cells by CAp24 FACS analysis. Shown are mean values obtained by challenging cells from two healthy donors with duplicate conditions