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Table 2 Lack of HIV-1 replication in PHA-activated CD4+T lymphocytes challenged with different exosome preparations

From: Latent HIV-1 is activated by exosomes from cells infected with either replication-competent or defective HIV-1

Days post-challenge input CAp24 pg/ml % of HIV-1+ cells
2 5 7 2 5 7
Exo U937 <15 <15 <15 <1 <1 <1
Exo U937 Δnef HIV-1 31 <15 <15 <1 <1 <1
Exo U937 Δnef
HIV-1 + wtNef
26 <15 <15 <1 <1 <1
Exo U937 Δnef
HIV-1 + NefG2A
44 <15 <15 <1 <1 <1
Exo CD4+ T lymphocytes <15 <15 <15 <1 <1 <1
Exo CD4+ T lymphocytes + HIV-1 <15 <15 <15 <1 <1 <1
Exo MDMs <15 <15 <15 <1 <1 <1
Exo MDMs + HIV-1 <15 <15 <15 <1 <1 <1
Exo Hut-78 <15 <15 <15 <1 <1 <1
Exo Hut-78/F12 89 <15 <15 <1 <1 <1
HIV-1, 50 pg <15 9980 2400 <1 32.2 7.9
  1. A total of 5 × 104 CD4+ T lymphocytes were spinoculated with 120 μU of the exosome preparations indicated in the left column. As a control, the same number of cells was challenged with 50 pg CAp24 equivalents of the T-tropic NL4-3 HIV-1 strain. Cell cultures were washed 24 h later, and viral replication was monitored at the indicated days post-infection by measuring CAp24 levels in supernatants as well as scoring HIV-1 expressing cells by CAp24 FACS analysis. Shown are mean values obtained by challenging cells from two healthy donors with duplicate conditions