Skip to main content

Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Fig. 4 | Retrovirology

Fig. 4

From: Latent HIV-1 is activated by exosomes from cells infected with either replication-competent or defective HIV-1

Fig. 4

Set up of a system of HIV-1 latent infection. Untouched CD4+ T lymphocytes were purified from PBMCs of healthy donors and challenged by spinoculation with (VSV-G) Δenv HIV-1 in the presence or not of AZT. As a control, conditions with unchallenged CD4+ T lymphocytes (Ctrl) were also run. After 48 h, cells were extensively washed and then left in culture for additional 24 h. a Intracytoplasmic CAp24 FACS analysis of CD4+ T lymphocytes 72 h post-infection. The results are the mean values + SD from nine independent experiments carried out with duplicates. b Intracytoplasmic CAp24 FACS analysis of CD4+ T lymphocytes which, 72 h post-infection, were either activated for 24 h by the indicated doses of PMA+ 0.5 μg/μL of ionomycin (PMA) or left untreated. Ctrl: uninfected CD4+ T lymphocytes. The results are the mean values + SD from three independent experiments carried out with duplicates. In the bottom panels, shown are representative raw results obtained by the intracytoplasmic CAp24 FACS analysis of CD4+ T lymphocytes either uninfected (Ctrl), or latently infected with (VSV-G) Δenv HIV-1 in the presence or not of AZT, and treated for 24 h with PMA + ionomycin

Back to article page