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Fig. 7 | Retrovirology

Fig. 7

From: Fluorescent protein-tagged Vpr dissociates from HIV-1 core after viral fusion and rapidly enters the cell nucleus

Fig. 7

YFP-Vpr is not shed from immobilized viruses after detergent lysis and exposure to cytosolic extract. a Removal of the viral membrane with TX-100. VSVpp co-labeled with YFP-Vpr (green, core) and mCherry-ICAM (red, membrane) were diluted in PBS and bound to Cell-Tak™ coated chamber coverslips (Lab-Tek) for 30 min at 4 °C. Images were acquired before and after the addition of 0.1 % TX-100 for 1 min, which effectively removed the viral membrane, as seen by the loss of the mCherry-ICAM signal. b TX-100 treatment (0.1 %) of immobilized ASLVpp co-labeled with YFP-Vpr and Gag-imCherry resulted in loss of mCherry from a fraction (35 %) of particles that properly matured. c Naked cores remaining after TX-100 lysis of immobilized ASLVpp were washed with PBS and incubated with 0.5 mg of cytosolic extract from rhesus monkey liver cells diluted in 0.3 ml PBS for 50 min at 37 °C. In all panels, the same image fields are shown before and after TX-100 or cytosol treatment

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