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Fig. 6 | Retrovirology

Fig. 6

From: Fluorescent protein-tagged Vpr dissociates from HIV-1 core after viral fusion and rapidly enters the cell nucleus

Fig. 6

Retention of GFP-Vpr by purified HIV-1 cores. a Analyses of HIV-1 cores released upon centrifugation of concentrated HIV-1 particles through a layer of Triton X-100 detergent. Proteins in the fractions were concentrated by TCA precipitation and subjected to SDS-PAGE and immunoblotted for gp120, GFP, HIV-1 CA, and cyclophilin A (CypA). Numbered fractions were collected from the top to the bottom of the gradient. Viral cores sedimented to fractions 9–11. b Analysis of GFP-Vpr-labeled HIV-1 particles in parallel following centrifugation on a gradient lacking the detergent layer. Intact HIV-1 particles sedimented to fractions 2–5

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