Fig. 2
From: Wide distribution and ancient evolutionary history of simian foamy viruses in New World primates
Detection of plasma/serum antibodies to SFV from spider monkey (SFVasp) and marmoset monkey (SFVcja) using a combined antigen western blot (WB) assay. a Upper panel shows seroreactivity of representative neotropical primate samples to the combined NWM SFV antigens from spider monkey (asp Ateles species) and marmoset (cja Callithrix jacchus) tissue cultures; lower panel shows reactivity to crude cell lysate antigens from uninfected canine thymocytes (Cf2Th). b Upper panel shows seroreactivity of representative Neotropical primate samples to combined Old World monkey (AGM African green monkey) and ape (cpz chimpanzee) SFV antigens except lanes 1 and 2 are plasma samples from SFVagm- and SFVcpz-infected humans as positive controls; lower panel shows reactivity to crude cell lysate antigens from uninfected Cf2Th. Seroreactivity was defined as those specimens with reactivity specific to the diagnostic Gag doublet proteins in the combined viral antigens. Lane 3 is a pedigreed negative human plasma control