Fig. 2

Time kinetics of p24 gag protein expression, synthesis of viral transcripts and virus release. a HIV-1 Gag positive cells were detected by intracellular staining for p24-Gag protein at indicated time points following treatment of ACH-2 cells with SAHA, prostratin or TNF-α. b Multiply spliced HIV-1 RNA transcripts, and c Unspliced HIV-1 (Gag-pol) RNA transcripts were detected by Real time PCR at indicated time points. The average fold changes in RNA transcripts over time zero observed in two independent experiments done in triplicates is shown. RPLPO (large ribosomal protein) was included for normalization. d The amount of virus released in the supernatant was detected by p24 ELISA and the p24 value at time zero was subtracted from individual time points to calculate the new virus released over time. The average of two independent experiments done in triplicate is shown