Fig. 1

Dose dependent reversal of latent HIV-1 in ACH-2 cells by SAHA, prostratin and TNF-α. a ACH-2 cells were treated with different reactivating agents for 18 h and p24-Gag was estimated by intracellular staining. A representative figure denoting intracellular HIV-1 p24 Gag detected by flow cytometry (N = 4) is shown here. Viable cells were gated based on side and forward scatter dot plot and the p24 positive cells were detected by anti-p24-FITC antibody b The percentage of HIV-1 Gag-p24 positive cells from multiple experiments (N = 4) are shown, to demonstrate the dose dependent response of ACH-2 to different activating reagents. Error bars represent standard deviation. c Effect of combination of reactivating agents. For comparison of results across samples from multiple experiments, HIV reactivation observed upon PMA (100 nM) treatment minus background (DMSO) was considered as 100 %. Error bars represent standard deviation (N = 4). *p < 0.05 by student t test