Fig. 9
From: Conserved determinants of lentiviral genome dimerization
NC dependent dimerization is sensitive to the nature of the DIS loop. a, c Mutations in the loop and the stem of the DIS are colored red on the secondary structures of the SIVcpzTAN1 and HIV-1NL4-3 DIS within the intact leaders. Native gel NC titration results obtained for SIVcpzTAN1 RNA containing HIV-1 DIS loop (SIVcpzTAN1 5′-L/HIV-1NL4-3 DIS) (b), and the HIV-1NL4-3 leader containing the DIS loop residues of SIVcpzTAN1 (HIV-1NL4-3 5′-L/SIVcpzTAN1 DIS) (d) in PI buffer. Addition of 0, 5, 10 equivalents of NC to the respective retroviral mutants were probed using TB and TBM running buffers. Monomers (M) and dimers (D) are denoted. SIVcpzTAN1 NC binding induces a significant equilibrium shift toward the dimer of the SIVcpzTAN1 5′-L/HIV-1NL4-3 DIS RNA, whereas HIV-1NL4-3 NC does not induce a detectable shift in the equilibrium of the HIV-1NL4-3 5′-L/SIVcpzTAN1 DIS chimeric leader RNA