Fig. 3
From: Conserved determinants of lentiviral genome dimerization
The U5:AUG formation promotes retroviral 5′-leader dimerization. Native agarose gel electrophoresis data obtained for leader constructs (5′-LWT, 5′-LΔAUG, and 5′-LLR) of a HIV-1NL4-3, b SIVcpzUS, c SIVcpzTAN1, and d HIV-2ROD [after 1-h incubation in PI buffer and measured using TB (top panel) and TBM (lower panel) as the running buffer]. Monomer and dimer bands are denoted as M and D, respectively. As observed for analogous HIV-1NL4-3 leader constructs, truncations that inhibit U5:AUG formation (5′-LΔAUG) also inhibit dimerization, whereas those designed to favor U5:AUG formation (5′-LLR) promote or restore dimerization. Quantitative analysis of gels [56] afforded the following % dimer values (reported as mean ± standard deviation) for two TBM gels for 5′-LWT; 5′-LΔAUG; 5′-LLR: a 52 ± 3; 32 ± 2; 64 ± 5. b 29 ± 5; 17 ± 3; 34 ± 5. c 18 ± 1; 5 ± 1; 21 ± 5. d 14 ± 3; 5 ± 2; 20 ± 3