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Fig. 4 | Retrovirology

Fig. 4

From: The role of antigen presenting cells in the induction of HIV-1 latency in resting CD4+ T-cells

Fig. 4

Productive and latent infection in resting T-cells co-cultured with antigen presenting cell subsets. a Representative dot plot of CD4+ T-cells co-cultured with antigen presenting cell (APC) subpopulations day 5 post infection with NL(AD8)Δnef-EGFP. Non-proliferating (eFluor670hi), non-productively infected (EGFP−) T-cells were sorted 5 days following infection. b EGFP expression in the total cell cultures at day 5 post-infection was used as a measure of productive infection. c Total and post-integrated latency was stimulated from eFluor670hiEGFP− cells with anti-CD3/CD28 and IL-7 for 3 days and EGFP expression was quantified using flow cytometry. Representative dot plots show EGFP expression following stimulation of eFluor670hiEGFP− sorted CD4+ T-cells in the absence (-L8 plot; total latency) and presence (+L8 plot; post integration latency) of the integrase inhibitor L8. d The frequency of total and e post-integration latent infection in resting CD4+ T-cells following co-culture with APC subpopulations. f Correlation of the frequency of productive infection and post-integrated latency (+L8) in each APC-T-cell co-culture. Each condition is identified by a different color and/or symbol. For all other panels, columns represent the median and open circles represent results from individual donors. Significant differences between conditions was measured by Wilcoxon matched pairs signed rank test where *p ≤ 0.05

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