Fig. 3

Resting CD4⁺ T-cell stimulation following co-culture with antigen presenting cells. Resting CD4⁺ T-cells were labeled with the proliferation dye eFluor670 and co-cultured with one of seven antigen presenting cell (APC) subpopulations, including B-cells; monocyte subpopulations-CD14^hi and CD14^loCD16^hi; DC subpopulations- plasmacytoid (p)DC and myeloid (m)DC subpopulations—CD1c⁺, CD141⁺ and SLAN⁺, at a ratio of log 1 (10:1), 2 (100:1) or 3 (1000:1) T-cells : APC. T-cell stimulation was measured by quantification of the percentage of eFluor670^lo CD4⁺ T-cells from APC-T-cell co-cultures following 5 days of culture in the a absence (syngeneic) or b presence of staphylococcal enterotoxin B (SEB). c eFluor670 labeled, resting CD4⁺ T-cells were cultured alone, or with APC subpopulations at a ratio of 10:1 and infected with NL(AD8)Δnef-EGFP. At day 3 post-infection, CD4⁺ eFluor670^lo T-cells were measured. Columns represent the median, open circles represent individual donors, *p ≤ 0.05, as determined by Wilcoxon matched pairs signed rank test