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Fig. 1 | Retrovirology

Fig. 1

From: The role of antigen presenting cells in the induction of HIV-1 latency in resting CD4+ T-cells

Fig. 1

Monocyte induced latency in non-proliferating CD4+ T-cells. a Resting CD4+ T-cells were labeled with eFluor670 and cultured alone, with bulk myeloid (m)DC or bulk monocytes (mono) at a ratio of 10:1. Following 24 h of culture, APC-T-cell co-cultures were infected with NL(AD8)Δnef-EGFP. b At day 5 post-infection productive infection was determined by quantification of EGFP in total cells and, non-proliferating, non-productively infected (eFluor670hiEGFP−) cells were sorted using flow cytometry and reactivated to determine frequency of latent infection. c Sorted non-proliferating (eFluor670hiEGFP−) CD4+ T-cells were stimulated with phytohaemagglutinin (PHA) stimulated feeder PBMC for 5 days and EGFP quantified as a measure of HIV-1 latency. Columns represent the median of the log transformed values, open circles represent individual donors. *p ≤ 0.05, **p ≤ 0.005 as determined by paired students t-test

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