Table 1 Selection of mitochondrial-related proteins which showed at least a ±2.0-fold deregulation in Jurkat-Tat101 versus control cells
Gene | Protein | Tat72 vs TetOff | Tat101 vs TetOff | Biological process | Peptide sequence | X-corr |
|---|---|---|---|---|---|---|
PKM2 | Pyruvate kinase isozymes M1/M2 | 6.5 | 36.8 | Glycolysis | APIIAVTRNPQTAR EAEAAIYHLQLFEELRR FGVEQDVDMVFASFIR GSGTAEVELKK IYVDDGLISLQVK KGVNLPGAAVDLPAVSEKDIQDLK LDIDSPPITAR RFDEILEASDGIMVAR SVETLKEMIK TATESFASDPILYRPVAVALDTK TATESFASDPILYRPVAVALDTKGPEIR | 2.65 1.81 2.90 2.06 1.88 3.72 2.01 3.16 1.96 2.80 3.18 |
LDHB | l-lactate dehydrogenase (B chain) | −0.9 | 10.4 | Glycolysis | IVADKDYSVTANSK LKDDEVAQLKK SADTLWDIQK SADTLWDIQKDLKDL | 2.07 2.26 2.05 2.08 |
SH3BGRL3 | SH3 domain-binding glutamic acid-rich-like protein 3 | 9.9 | 8.1 | Oxidative stress | IQYQLVDISQDNALRDEMR VYSTSVTGSR | 3.12 2.48 |
PRDX1 | Peroxiredoxin-1 | 7.9 | 7.8 | Oxidative stress | QGGLGPMNIPLVSDPKR QITVNDLPVGR TIAQDYGVLKADEGISFR | 1.78 2.06 2.95 |
PDIA4 | Protein disulfide-isomerase A4 | 0.7 | 5.2 | Oxidative stress | FDVSGYPTIK FDVSGYPTLK IDATSASVLASR MDATANDVPSDR VDATAETDLAKR | 1.98 1.98 2.51 1.75 1.83 |
TKT | Transketolase | −1.2 | 4.9 | Energy metabolism | ILATPPQEDAPSVDIANIR KAYGQALAK MFGIDRDAIAQAVR | 1.80 2.29 2.71 |
TXNDC17 | Thioredoxin domain-containing protein 17 | 0.9 | 2.8 | Oxidative stress | YEEVSVSGFEEFHR | 1.77 |
SOD1 | Superoxide dismutase | 0.4 | 3.3 | Oxidative stress | TLVVHEKADDLGK TLVVHEKADDLGKGGNEESTK | 1.76 3.07 |
ALDOA | Fructose-bisphoshate aldolase A | 8.9 | 2.7 | Glycolysis | AAQEEYVKR GILAADESTGSIAK GILAADESTGSIAKR IGEHTPSALAIMENANVLAR IVAPGKGILAADESTGSIAK IVAPGKGILAADESTGSIAKR LQSIGTENTEENR | 1.88 3.03 2.61 2.69 3.80 3.91 2.41 |
P4HB | Protein disulfide-isomerase | 1.5 | 2.5 | Oxidative stress | MDSTANEVEAVKVHSFPTLK | 2.02 |
GYS1 | Glycogen synthase, muscle | 0.9 | 2.5 | Glycogenesis | LSDLLDWK TQVELLEAPTPALKR VGGIYTVLQTK | 1.79 1.94 1.87 |
TPM4 | Tropomyosin alpha-4 | 9.9 | 2.2 | Oxidative stress | KIQALQQQADEAEDR | 2.13 |
TALDO1 | Transaldolase | 0.6 | 2.2 | Energy metabolism | WLHNEDQMAVEK | 2.15 |
PEBP1 | Phosphatidylethanolamine-binding protein | 0.0 | 2.2 | Oxidative stress | LYEQLSGK WSGPLSLQEVDEQPQHPLHVTYAGAA | 1.79 2.68 |
PARK7 | Protein DJ-1 | 1.8 | −2.0 | Autophagy Fusion Morphogenesis | GAEEMETVIPVDVMR GAEEMETVIPVDVMRR | 2.08 2.00 |
PGK1 | Phosphoglycerate kinase 1 | 1.3 | −2.7 | Glycolysis | AHSSMVGVNLPQK LGDVYVNDAFGTAHR VDFNVPMKNNQITNNQR VLPGVDALSNI | 1.81 2.60 2.00 1.96 |
TPI1 | Triosephosphate isomerase isoform 1 | −5.3 | −3.0 | Glycolysis gluconeogenesis | SNVSDAVAQSTR VVLAYEPVWAIGTGK | 2.95 2.65 |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | −10.8 | −11.4 | Glycolysis | GALQNIIPASTGAAK IKWGDAGAEYVVESTGVFTTMEK LISWYDNEFGYSNR VGVNGFGR VVDLMAHMASKE WGDAGAEYVVESTGVFTTMEK | 1.81 2.17 3.07 2.09 2.87 2.53 |
PDIA3 | Protein disulfide-isomerase A3 | −15.0 | −11.9 | Oxidative stress | DGEEAGAYDGPR EATNPPVIQEEKPK ELSDFISYLQR GFPTIYFSPANK IFRDGEEAGAYDGPR KYEGGRELSDFISYLQR LSKDPNIVIAK MDATANDVPSPYEVR QAGPASVPLR QAGPASVPLRTEEEFKK RLAPEYEAAATR TADGIVSHLKK YGVSGYPTLK | 2.09 2.30 2.43 2.34 3.09 2.91 2.14 2.62 2.59 1.82 2.68 2.68 2.05 |