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Table 1 Selection of mitochondrial-related proteins which showed at least a ±2.0-fold deregulation in Jurkat-Tat101 versus control cells

From: Intracellular expression of Tat alters mitochondrial functions in T cells: a potential mechanism to understand mitochondrial damage during HIV-1 replication

Gene Protein Tat72 vs TetOff Tat101 vs TetOff Biological process Peptide sequence X-corr
PKM2 Pyruvate kinase isozymes M1/M2 6.5 36.8 Glycolysis APIIAVTRNPQTAR
EAEAAIYHLQLFEELRR
FGVEQDVDMVFASFIR
GSGTAEVELKK
IYVDDGLISLQVK
KGVNLPGAAVDLPAVSEKDIQDLK
LDIDSPPITAR
RFDEILEASDGIMVAR
SVETLKEMIK
TATESFASDPILYRPVAVALDTK
TATESFASDPILYRPVAVALDTKGPEIR
2.65
1.81
2.90
2.06
1.88
3.72
2.01
3.16
1.96
2.80
3.18
LDHB l-lactate dehydrogenase (B chain) −0.9 10.4 Glycolysis IVADKDYSVTANSK
LKDDEVAQLKK
SADTLWDIQK
SADTLWDIQKDLKDL
2.07
2.26
2.05
2.08
SH3BGRL3 SH3 domain-binding glutamic acid-rich-like protein 3 9.9 8.1 Oxidative stress IQYQLVDISQDNALRDEMR
VYSTSVTGSR
3.12
2.48
PRDX1 Peroxiredoxin-1 7.9 7.8 Oxidative stress QGGLGPMNIPLVSDPKR
QITVNDLPVGR
TIAQDYGVLKADEGISFR
1.78
2.06
2.95
PDIA4 Protein disulfide-isomerase A4 0.7 5.2 Oxidative stress FDVSGYPTIK
FDVSGYPTLK
IDATSASVLASR
MDATANDVPSDR
VDATAETDLAKR
1.98
1.98
2.51
1.75
1.83
TKT Transketolase −1.2 4.9 Energy metabolism ILATPPQEDAPSVDIANIR
KAYGQALAK
MFGIDRDAIAQAVR
1.80
2.29
2.71
TXNDC17 Thioredoxin domain-containing protein 17 0.9 2.8 Oxidative stress YEEVSVSGFEEFHR 1.77
SOD1 Superoxide dismutase 0.4 3.3 Oxidative stress TLVVHEKADDLGK
TLVVHEKADDLGKGGNEESTK
1.76
3.07
ALDOA Fructose-bisphoshate aldolase A 8.9 2.7 Glycolysis AAQEEYVKR
GILAADESTGSIAK
GILAADESTGSIAKR
IGEHTPSALAIMENANVLAR
IVAPGKGILAADESTGSIAK
IVAPGKGILAADESTGSIAKR
LQSIGTENTEENR
1.88
3.03
2.61
2.69
3.80
3.91
2.41
P4HB Protein disulfide-isomerase 1.5 2.5 Oxidative stress MDSTANEVEAVKVHSFPTLK 2.02
GYS1 Glycogen synthase, muscle 0.9 2.5 Glycogenesis LSDLLDWK
TQVELLEAPTPALKR
VGGIYTVLQTK
1.79
1.94
1.87
TPM4 Tropomyosin alpha-4 9.9 2.2 Oxidative stress KIQALQQQADEAEDR 2.13
TALDO1 Transaldolase 0.6 2.2 Energy metabolism WLHNEDQMAVEK 2.15
PEBP1 Phosphatidylethanolamine-binding protein 0.0 2.2 Oxidative stress LYEQLSGK
WSGPLSLQEVDEQPQHPLHVTYAGAA
1.79
2.68
PARK7 Protein DJ-1 1.8 −2.0 Autophagy
Fusion
Morphogenesis
GAEEMETVIPVDVMR
GAEEMETVIPVDVMRR
2.08
2.00
PGK1 Phosphoglycerate kinase 1 1.3 −2.7 Glycolysis AHSSMVGVNLPQK
LGDVYVNDAFGTAHR
VDFNVPMKNNQITNNQR
VLPGVDALSNI
1.81
2.60
2.00
1.96
TPI1 Triosephosphate isomerase isoform 1 −5.3 −3.0 Glycolysis gluconeogenesis SNVSDAVAQSTR
VVLAYEPVWAIGTGK
2.95
2.65
GAPDH Glyceraldehyde-3-phosphate dehydrogenase −10.8 −11.4 Glycolysis GALQNIIPASTGAAK
IKWGDAGAEYVVESTGVFTTMEK
LISWYDNEFGYSNR
VGVNGFGR
VVDLMAHMASKE
WGDAGAEYVVESTGVFTTMEK
1.81
2.17
3.07
2.09
2.87
2.53
PDIA3 Protein disulfide-isomerase A3 −15.0 −11.9 Oxidative stress DGEEAGAYDGPR
EATNPPVIQEEKPK
ELSDFISYLQR
GFPTIYFSPANK
IFRDGEEAGAYDGPR
KYEGGRELSDFISYLQR
LSKDPNIVIAK
MDATANDVPSPYEVR
QAGPASVPLR
QAGPASVPLRTEEEFKK
RLAPEYEAAATR
TADGIVSHLKK
YGVSGYPTLK
2.09
2.30
2.43
2.34
3.09
2.91
2.14
2.62
2.59
1.82
2.68
2.68
2.05
  1. Proteins were detected by mass spectrometry in total protein extracts. All peptides detected showed 95 % probability for protein expression and a minimum cross-correlation (X-Corr) value of 1.75