Fig. 7
Effect of intracellular Tat expression on cellular polarization and the expression of cytoskeleton-related proteins. a Network of predicted interactions between cytoskeletal network proteins deregulated in Jurkat-Tat101 vs control cells (as specified in Table 3). Medium confidence score level was chosen (0.400). Data supporting protein–protein interactions derived from experimental studies (dark purple lines), homology (light purple lines), databases (light blue lines), text mining (light green lines), concurrence (dark blue lines) and co-expression (black lines). Node colour is arbitrary. b Rac1 and RhoA GTPases activations were measured using a luminescence-based assay. Data shown are absolute RLUs from three independent experiments. c Cdc42 GTPase activation was measured in protein extracts using a colorimetric-based assay. Data shown are absolute absorbance at 490 nm from three independent experiments. d Cellular polarization was studied by immunofluorescence in Jurkat-Tat72, Jurkat-Tat101 and control cells adhered to fibronectin using an antibody against α-tubulin and a secondary antibody conjugated with Alexa-555. e Analysis of migration capacity in Jurkat-Tat72 and Jurkat-Tat101 in comparison with control cells in the absence of any migratory stimuli. The relative increase of migrated events in comparison with control cells from three independent experiments is shown. Statistical significance was calculated with Kruskal–Wallis test with Dunn’s multiple comparison test (***p < 0.001 vs control)