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Fig. 5 | Retrovirology

Fig. 5

From: Intracellular expression of Tat alters mitochondrial functions in T cells: a potential mechanism to understand mitochondrial damage during HIV-1 replication

Fig. 5

Effect of Tat on the transcription of mt-DNA and fusion/fission genes. a qPCR analysis of mRNAs levels from the mtDNA-encoded genes COX-II, MTND-2, MTND-5, MTND-6, MT-CYB and MTCO-3. b qRT-PCR analysis of mRNAs levels from nuclear DNA-encoded genes TFAM and NRF1. c qRT-PCR analysis of nuclear-encoded mRNAs from MFN2 and DNM1L genes, which are involved in mitochondria fusion and fission, respectively. Total mRNA from control cells, Jurkat-Tat72, and Jurkat-Tat101 cells was analyzed. Nuclear S18 mRNA expression was used as house-keeping gene. All data shown are media and SEM from at least three independent experiments. Statistical significance was calculated by Kruskal–Wallis test with Dunn’s multiple comparison post hoc analysis (*p < 0.05, **p < 0.01 ***p < 0.001 vs control)

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Retrovirology

ISSN: 1742-4690

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