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Fig. 2 | Retrovirology

Fig. 2

From: Intracellular expression of Tat alters mitochondrial functions in T cells: a potential mechanism to understand mitochondrial damage during HIV-1 replication

Fig. 2

Quantification of citrate synthase activity, mitochondria respiratory capacity and lactate levels in Jurkat-Tat101 cells. a Activity of the citrate synthase measured with commercial enzymatic assays in Jurkat-Tat72 (JJ-Tat72), Jurkat-Tat101 (JJ-Tat101) vs control cells. One citrate unit is equivalent to a μmole/mL/min. b ATP production was measured using a chemiluminescence-based assay. c Activity of the complex-I and complex-V of the respiratory chain was measured in Jurkat-Tat72, Jurkat-Tat101 and control cells in mg/mL/min and it was normalized according to mitochondria amount indirectly measured as the activity of the citrate synthase enzyme. d Lactate levels were measured in intracellular and supernatant samples and concentration was expressed in ng/μl (left and right panels, respectively) from Jurkat-Tat72, Jurkat-Tat101 vs control cells. All data shown are media and standard error of the mean (SEM) from at least three independent experiments. Statistical significance was calculated by Kruskal–Wallis test and post hoc multiple comparisons were performed with Dunn’s multiple comparison analysis (*p < 0.05 and **p < 0.01 vs control)

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