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Fig. 1 | Retrovirology

Fig. 1

From: Furin-dependent CCL17-fused recombinant toxin controls HTLV-1 infection by targeting and eliminating infected CCR4-expressing cells in vitro and in vivo

Fig. 1

Elimination of HTLV-1-infected cell lines by TARC–PE38. a HTLV-1-infected S1T, TL-Om1, MT-2, and HUT-102 cells and uninfected Jurkat, Raji, U937, and THP-1 cells were tested for their expression of CCR4 with flow cytometry without (solid red line) or with an anti-CCR4 antibody (solid line filled with black). MFI, mean fluorescence intensity. b To examine the killing effects of TARC–PE38 on the cells described above, the numbers of live cells were counted daily for 5 days after either 10 or 20 μg/ml TARC–PE38 (closed triangles), 10 or 20 μg/ml TARC–OFA (open circles), or PBS (closed circles) was added to each cell line. The data are expressed as means ± SD (×105/ml). c To further determine the cytotoxicity of TARC–PE38 in the cells described above, each cell line was incubated with either TARC–PE38 (closed triangles) or TARC–OFA (open circles) at doses of 0 (PBS only added), 5, 10, or 20 μg/ml for 5 days, and the numbers of live cells were then counted. The data are expressed as means ± SD (×105/ml). d To rule out nonspecific effects of PE38, either PBS (closed circles) or 20 μg/ml PE38 alone (closed triangles) was added to S1T cells. Then the numbers of live cells were counted daily for 5 days, and the data are expressed as means ± SD (×105/ml). These results are representative of four independent experiments

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