Fig. 5

Intracellular Gag is reduced in HAP1 KO cell line. a Cells were transfected with plasmids able to produce pseudotyped HIV. 48 h after transfection, the supernatant was removed and cells were lysed for Western blot analyses. GAPDH was a loading control. Viral Gag products are labelled at the left. b Quantitation of Western blots shown in a using Image J. The total p24-associated viral products were quantified and the sum normalised to that of GAPDH preceded by normalisation to WT. Error bar represents SEM from five replicates and statistics were done by using a paired two-tailed Student t test; statistical significance is shown by the asterisk.